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KMID : 0379119940220020122
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Korean Journal of Mycology
1994 Volume.22 No. 2 p.122 ~ p.129
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Study on the Role of Metal ions for the Activity of the Mitochondrial F1-ATPase in Lentinus edodes
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Abstract
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The role of metal ions for the activity of the mitochondrial F₁-ATPase was studied. Removal of non-heme iron ion from the mitochondria by dialysis against chelating agents, 10 mM ethylenediaminetetraacetic acid(EDTA) and 10 mM o-phenanthroline(o-Phe), led to 56% and 49% inactivation of the enzyme, respectively. The enzyme dialyzed against EDTA was reactivated 81% by the addition of 0.5 mM Fe^(3+) and 70% by 0.5 mM Mg^(2+). But, Fe^(2+) did not reactivate the enzyme. Coexistence of 0.5 mM Fe^(2+) and 0.5 mM Mg^(2+) resulted in 95% reactivation of the enzyme, while Fe^(3+) with 0.5 mM Mg^(2+) did not reactivate the enzyme like the effect of Fe^(2+) alone. The enzyme dialyzed against o-Phe showed the similar results. These data showed that Fe^(3+) is predominantly required for the activity of the mitochondrial F₁-ATPase in Lentinus edodes and stimulated the activity of it by Mg^(2+). Fe^(3+) and Mg^(2+) increased enzyme´s affinity for substrate, decreasing the Km value 1.67 mM to 0.65 mM.
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